Abstract:
Objective To investigate whether transforming growth factor-beta 1(TGF-β1) is involved in the process of carbon tetrachloride(CCl
4)-induced acute chemical liver injury.
Methods Acute liver injury in mouse was induced by intraperitoneal injection of CCl
4.The level of serum TGF-β1 in mouse was detected with enzyme-linkde immunosorbent assay(ELISA).The expression of TGF-β1 signaling molecules Smad2,3 mRNA was examined with reverse transcriptase PCR(RT-PCR).
Results Both levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and TGF-β1 in serum of mouse increased significantly at 1st and 3rd date after CCl
4 treatment (
P < 0.01 for all).RT-PCR results showed that the expression of TGF-β1 mRNA in liver was up-regulated significantly in CCl
4-treated mouse compared with that in the control mouse treated with olive oil,and the expressions of TβRII,signaling molecules Smad2,3 mRNA were also obviously up-regulated.
Conclusion Levels of TGF-β1 and signaling molecules Smad2,Smad3 increase during CCl
4-induced acute chemical liver injury in mouse,which suggest that TGF-β1 is involved in the process of CCl
4-induced acute chemical liver injury.