Abstract: Objective To evaluate the relationship between diet and oral cancer in males.Methods A case-control study was carried out between September 2010 and December 2014,in which 313 male oral cancer patients confirmed by pathological diagnosis and 575 controls were matched in gender and age.All participants were personally interviewed with a standard questionnaire.Results The cases had significantly lower proportions of consuming following food at the frequency of <1 time/day than the controls:poultry (1.28% vs.6.78%),fish (15.65% vs.43.68%),seafood (7.35% vs.24.87%),green vegetables (74.44% vs.94.61%),non-green leafy vegetables (69.97% vs.93.22%),and fruits (11.50% vs.34.43%) and without regular intake of health care products (3.83% vs.12.17%),without regular intake of vitamin supplements (2.56% vs.10.61%),drinking coffee (0.96% vs.4.52%),and eating fruits at the frequency of <1 time/day (46.01% vs.51.48%),respectively (P < 0.05 for all).Multivariate unconditional logistic regression analysis showed that eating fish ≥1 time/day,eating seafood ≥1 time/day,eating green vegetables ≥1 time/day,eating green vegetables ≥1 time/day,eating fruits<1 time/day,eating fruits≥1 time/day,regular intake of health care products,and regular intake of vitamin supplements were the protective factors for oral cancer in the males,while dietary preference to salty food was a risk factors.Conclusion Consumption of fish,seafood,green leafy and non-green leafy vegetables,fruits,health care products and vitamin supplements and dietary preference to salty food may have impact on the incidence of oral cancer in males at a certain extent.