Abstract:
Objective To study hepatoprotective effect of
Pyracantha fortuneana polysaccharides on liver injury induced by carbon tetrachloride (CCI
4) in rats.
Methods Fifty Wistar rats were randomly divided into five groups:control group,CCI
4 group,biphenyldicarboxylate pills (BP) group,and 200 and 400 mg/kg
Pyracantha fortuneana polysaccharides groups.The rats of control group were given olive oil by intraperitoneal injection (IP) and those of other groups were given 30% CCI
4 by IP,respectively.The rats of BP group were given BP (200 mg/kg) by gavage and the rats of
Pyracantha fortuneana polysaccharides groups were given
Pyracantha fortuneana polysaccharides of 200 and 400 mg/kg by gavage,respectively.Five weeks after the treatments,the contents of serum alanine transferase (ALT) and aspartate transaminase (AST) were determined; the levels of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) and the activity of total antioxidative capacity (T-AOC) in liver were measured.The mRNA expression levels of nuclear factor erythroid 2-related factor (Nrf2) and catalase (CAT) were detected with real time PCR.
Results The serum ALT (221.13±28.46 and 190.31 ±27.32 U/L)and AST (286.4±31.57 and 224.6±30.52 U/L)decreased remarkably in the rats of 200 and 400 mg/kg
Pyracantha fortuneana polysaccharides groups compared to those of the rats in CCI
4 group (521.42±68.21 and 589.17±39.44 U/L) (all
P<0.05).The MDA (23.29±4.38 and 18.67±3.28 nmol/mg pro) in liver of the rats of 200 and 400 mg/kg
Pyracantha fortuneana polysaccharides groups decreased remarkably compared to that of rats in the CCI
4 group (both
P<0.05).The T-AOC (106.32±14.23 and 152.31±16.12 U/mL) in liver of the rats in 200 and 400 mg/kg
Pyracantha fortuneana polysaccharides groups increased significantly compared to that (73.15±9.23 U/mL)of the rats in CCI
4 group (both
P<0.05).The mRNA expression levels of Nrf2 and CAT increased in the liver of rats in
Pyracantha fortuneana polysaccharides groups compared with those of the rats in CCI
4 group.
Conclusion Pyracantha fortuneana polysaccharides administration is effective in attenuating CCI
4-induced liver injury and the effects may be related to the enhancement of antioxidant activity of
Pyracantha fortuneana polysaccharides in rats.