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沈楠, 黄晓东, 王艳春, 齐玲, 徐博, 陈雪, 安英, 刘婷婷. 萱草花黄酮对免疫性肝损伤小鼠保护作用及机制[J]. 中国公共卫生, 2017, 33(2): 202-205. DOI: 10.11847/zgggws2017-33-02-08
引用本文: 沈楠, 黄晓东, 王艳春, 齐玲, 徐博, 陈雪, 安英, 刘婷婷. 萱草花黄酮对免疫性肝损伤小鼠保护作用及机制[J]. 中国公共卫生, 2017, 33(2): 202-205. DOI: 10.11847/zgggws2017-33-02-08
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SHEN Nan, HUANG Xiao-dong, WANG Yan-chun.et al, . Protective effect of Hemerocallis citrina baroni flavonids on autoimmune liver injury and its mechanism in mice[J]. Chinese Journal of Public Health, 2017, 33(2): 202-205. DOI: 10.11847/zgggws2017-33-02-08
Citation: SHEN Nan, HUANG Xiao-dong, WANG Yan-chun.et al, . Protective effect of Hemerocallis citrina baroni flavonids on autoimmune liver injury and its mechanism in mice[J]. Chinese Journal of Public Health, 2017, 33(2): 202-205. DOI: 10.11847/zgggws2017-33-02-08
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萱草花黄酮对免疫性肝损伤小鼠保护作用及机制

Protective effect of Hemerocallis citrina baroni flavonids on autoimmune liver injury and its mechanism in mice

    摘要
  • 摘要: 目的 探讨萱草花黄酮对免疫性肝损伤小鼠保护作用及凋亡相关基因bax、bcl-2表达变化。方法 使用卡介苗和脂多糖制备小鼠免疫性肝损伤模型，将60只昆明小鼠随机分为对照组、模型组、低、中、高剂量萱草花黄酮（15、30、60 mg/kg）组。苏木素-伊红染色观察肝组织病理损伤程度；比色法测定肝组织中超氧化物歧化酶（SOD）、丙二醛和谷胱甘肽过氧化物酶（GSH-Px）含量；生化分析仪检测血清中谷丙转氨酶（ALT）含量；免疫组化法检测肝脏bax、bcl-2表达。结果 与对照组比较，模型组小鼠血清中ALT水平（105.48±46.34）U/L、丙二醛含量（8.57±0.54）nmol/mgprot明显升高，SOD与GSH-Px活力分别为（168.14±13.21）、（95.32±1.48）U/mgprot明显下降（P<0.01）；与模型组比较，30、60 mg/kg萱草花黄酮组小鼠血清中ALT水平分别为（56.38±26.31）、（59.77±13.34）U/L、丙二醛含量分别为（5.37±0.34）、（5.52±0.38）nmol/mgprot降低（P<0.05），SOD与GSH-Px活力分别为（197.40±25.22）、（249.41±24.08）与（107.32±1.83）、（114.24±1.41）U/mgprot升高（P<0.05）；与对照组比较，模型组小鼠肝脏bax基因表达上调，bcl-2基因表达下调（P<0.05）；与模型组比较，萱草花黄酮小鼠肝脏bax表达下调，bcl-2表达上调（P<0.05）。结论 萱草花黄酮对小鼠免疫性肝损伤具有一定保护作用，其机制可能与提高小鼠抗氧化能力、下调促凋亡基因bax表达、上调抗凋亡基因bcl-2表达有关。

     

    Abstract: Objective To study protective effect of Hemerocallis citrina baroni flavonids (HCBF) on autoimmune liver injury and the influence of HCBF on expressions of BCL-2-associated X protein (bax) and B cell lymyhoma/leukemia-2 protein (bcl-2) in mice.Methods The diabetic nephropathy (DN) mice model was established by injections of Bacillus Calmette-Guerin (BCG) and lipopolysaccharide (LPS).Sixty male Kunming mice were randomly divided into five groups:a control group,a DN model gourp,and low,moderate,and high treatment groups with HCBF of 15,30,60 mg/kg,respectively.Liver pathological changes were observed with hematoxylin-eosin staining.The contents of superoxide dismutase (SOD),malondialdehyde (MDA) and glutathione-peroxidase (GSH-Px) were measured with colorimetry.Serum alanine aminotransferase (ALT) was detected with biochemistry analyzer.The expressions of bax and bcl-2 were determined with immunohistochemistry.Results Compared to those of the controls,serum ALT (105.48±46.34 U/L) and MDA (8.57±0.54 nmol/mgprot) increased but SOD (168.14±13.21 U/mgprot) and GST-Px (95.32±1.48 U/mgprot) decreased in the mice of model group significantly (P<0.01 for all).Compared to those of the mice of the model group,serum ALT (56.38±26.31 and 59.77±13.34 U/L) and MDA (5.37±0.34 and 5.52±0.38 nmol/mgprot) decreased but SOD (197.40±25.22 and 249.41±24.08 nmol/mgprot) and GST-Px (107.32±1.83 and 114.24±1.41 U/mgprot) increased significantly for the mice of 30 and 60 mg/kg HCBF treatment groups (P<0.05 for all).Compared to the mice in the control,the expression of bax was up-regulated but the expression of bcl-2 was down-regulated for the mice in the model group (both P<0.05).Compared to the mice in the model group,the expression of bax was down-regulated but the expression of bcl-2 was up-regulated for the mice in the HCBF treatment groups (P<0.05 for all).Conclusion HCBF exhibits protective effect on autoimmune liver injury in mice and the effect may relate to the enhancement of antioxidant capacity,downregulation of bax and upregulation of bcl-2 in the mice.

     

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