补肾活血中药对肾小球肾炎大鼠肾组织TGF-_1及CTGF表达影响

刘敏; 谭小月; 张勉之

doi:10.11847/zgggws2017-33-03-35

补肾活血中药对肾小球肾炎大鼠肾组织TGF-_1及CTGF表达影响

Effect of Chinese medicine for invigorating kidney and promoting blood circulation on expressions of TGF-_1 and CTGF in renal tissues of rats with glomerular nephritis

摘要

摘要: 目的探讨补肾活血中药对肾小球肾炎大鼠肾组织转化生长因子-β1（TGF-β_1）及结缔组织生长因子（CTGF）表达的影响。方法选取6月龄Wister健康雄性大鼠40只，对其中30只大鼠建立肾小球肾炎大鼠模型，并将此30只造模成功的大鼠随机分为模型组、中药组和盐酸贝那普利组，每组10只，选取另10只未建模大鼠作为对照组；对照组和模型组均给予等量生理盐水灌胃，中药组给予补肾活血中药灌胃，盐酸贝那普利组给予西药盐酸贝那普利灌胃，治疗8周后，测定各组24h尿蛋白、血尿素氮和血肌酐水平，处死后采用免疫荧光化学法检测TGF-β_1和CTGF蛋白相对表达量。结果干预过程中模型组有1只大鼠死亡；模型组、中药组和盐酸贝那普利组大鼠24h尿蛋白分别为（96.58±10.32）、（49.76±9.82）和（50.30±10.02）mg/24h，血尿素氮分别为（10.82±0.59）、（5.98±0.57）和（6.03±0.52）mmol/L，血肌酐分别为（33.54±5.32）、（17.89±5.17）和（17.96±5.03）μmol/L，均高于对照组的（10.38±2.17）mg/24h、（4.01±0.37）mmol/L、（2.55±0.71）μmol/L（均P< 0.05），且中药组和盐酸贝那普利组24 h尿蛋白、血尿素氮和血肌酐水平均低于模型组（均P< 0.05），而中药组和盐酸贝那普利组各指标水平差异均无统计学意义（均P> 0.05）；模型组、中药组和盐酸贝那普利组大鼠TGF-β_1免疫荧光相对含量分别为（5.296±0.124）、（0.568±0.156）和（1.697±0.468），CTGF免疫荧光相对含量分别为（5.347±1.285）、（1.485±0.466）和（1.078±0.327），均高于对照组的（0.035±0.007）和（0.017±0.004）（均P< 0.05），且中药组和盐酸贝那普利组TGF-β_1和CTGF免疫荧光相对含量均低于模型组（均P< 0.05），中药组TGF-β_1免疫荧光相对含量低于盐酸贝那普利组（t=7.237，P< 0.001），而2组CTGF免疫荧光相对含量差异无统计学意义（P> 0.05）。结论补肾活血中药可明显降低肾小球肾炎大鼠实验室相关指标，抑制TGF-β_1和CTGF表达水平。

Abstract: ObjectiveTo observe the effect of the Chinese medicine for invigorating kidney and promoting blood circulation on expressions of transforming growth factor-β_1 (TGF-β_1) and connective tissue growth factor (CTGF) in renal tissues of the rats with glomerular nephritis.MethodsThirty healthy male Wister rats aged 6 months were adopted to construct a animal glomerular nephritis model and then randomly divided into a model group (MG),Chinese medicine group (CMG) and benazepril group (BG);another 10 rats of the same batch were used as a control group (CG).The rats of CMG were gavaged with a Chinese medicine for invigorating kidney and promoting blood circulation (a decoction composed of rhbarb,caterpillar fungus,salvia,astragalus,and Artemisia capillaris) once a day for 8 weeks;the rats of BG were administered with benazepril and those of MG and CG with normal saline the same way as for the CMG.By the end of the treatments,the protein content in 24 hours urine (24hU),the concentrations of blood urea nitrogen (BUN) and serum creatinine (Scr) were detected;the relative expressions of TGF-β_1 and CTGF were determined with immunofluorescence technique.ResultsSignificantly higher protein content of 24hU (96.58±10.32,49.76±9.82,and 50.30±10.02 mg),concentration of BUN (10.82±0.59,5.98±0.57,and 6.03±0.52 mmol/L),and the concentration of Scr (33.54±5.32,17.89±5.17,and 17.96±5.03μmol/L) were observed in the rats of MG,CMG,and BG compared to those (10.38±2.17 mg,4.01±0.37 mmol/L,and 2.55±0.71μmol/L) in the rats of CG (all P <0.05);the content of 24hU and the concentrations of BUN and Scr of CMG and BG were significantly lower than those of the MG (all P <0.05);while,there were no significant differences in the content of 24hU and the concentrations of BUN and Scr between the rats of CMG and BG (all P >0.05).For the rats of MG,CMG,and BG,the relative expression of TGF-β_1 was 5.296±0.124,0.568±0.156,and 1.697±0.468 and that of CTGF was 5.347±1.285,1.485±0.466,and 1.078±0.327,respectively,and all were higher significantly compared to those (0.035±0.007 and 0.017±0.004) in the rats of CG (all P <0.05);the relative expressions of TGF-β_1 and CTGF of the CMG and BG were significantly lower than those of the MG (all P<0.05);the expression of TGF-β_1 of CMG was significantly lower than that of BG (t=7.237，P< 0.001),but there was no significant difference in the expression of CTGF between the two groups (both P <0.05).ConclusionThe Chinese medicine for invigorating kidney and promoting blood circulation can significantly reduce laboratory indexes and inhibit expressions of TGF-β_1 and CTGF related to glomerular nephritis in rats.

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