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miR-155对喉鳞状细胞癌Hep2细胞增殖影响及机制

王轶 周玉琳 覃泽平

王轶, 周玉琳, 覃泽平. miR-155对喉鳞状细胞癌Hep2细胞增殖影响及机制[J]. 中国公共卫生, 2017, 33(7): 1070-1074. doi: 10.11847/zgggws2017-33-07-10
引用本文: 王轶, 周玉琳, 覃泽平. miR-155对喉鳞状细胞癌Hep2细胞增殖影响及机制[J]. 中国公共卫生, 2017, 33(7): 1070-1074. doi: 10.11847/zgggws2017-33-07-10
WANG Yi, ZHOU Yu-lin, QIN Ze-ping. Effect and mechanism of miR-155 on proliferation of laryngeal squamous cell carcinoma Hep2 cell line[J]. Chinese Journal of Public Health, 2017, 33(7): 1070-1074. doi: 10.11847/zgggws2017-33-07-10
Citation: WANG Yi, ZHOU Yu-lin, QIN Ze-ping. Effect and mechanism of miR-155 on proliferation of laryngeal squamous cell carcinoma Hep2 cell line[J]. Chinese Journal of Public Health, 2017, 33(7): 1070-1074. doi: 10.11847/zgggws2017-33-07-10

miR-155对喉鳞状细胞癌Hep2细胞增殖影响及机制

doi: 10.11847/zgggws2017-33-07-10
详细信息
    作者简介:

    王轶(1974-),男,四川南充人,副主任医师,博士,研究方向:耳鼻咽喉临床。

  • 中图分类号: R114

Effect and mechanism of miR-155 on proliferation of laryngeal squamous cell carcinoma Hep2 cell line

  • 摘要: 目的 探讨miR-155对喉鳞状细胞癌Hep2细胞增殖影响及具体机制。方法 采用miR-155 NC和miR-155 inhibitor转染Hep2细胞,realtime-PCR法检测转染效果,细胞计数盒-8(CCK-8)法检测不同转染时间(12、24、36、48、60、72 h)细胞活力,流式细胞术检测细胞凋亡及细胞周期;Western blot检测Hep2细胞中细胞周期蛋白A1(cyclin A1)、cyclin D1、磷脂酰肌醇3激酶/蛋白激酶B/叉形头转录因子(Foxo3a)信号通路相关蛋白表达量。结果 miR-155 inhibitor组中miR-155表达量(0.34±0.03)明显低于miR-155 NC组(1.25±0.10),且转染24、36、48、60、72 h后,miR-155 inhibitor组Hep2细胞活力明显低于miR-155 NC组;与miR-155 NC组比较,miR-155 inhibitor组Hep2细胞凋亡率明显提高,细胞周期阻滞于G1期,cyclin A1、cyclin D1、PI3K、p-AKT表达下调,Foxo3a表达上调,差异均有统计学意义(均P<0.01)。结论 miR-155表达下调可降低Hep2细胞活力、增加细胞凋亡率,其机制可能与miR-155调控PI3K/AKT/Foxo3a信号通路有关。
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出版历程
  • 接收日期:  2017-02-10
  • 刊出日期:  2017-07-10

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