Abstract: Objective To study the effect of mindfulness intervention on negative emotion and interpersonal acceptance ability in nurse trainees with left-behind experience.Methods Between January 2015 and January 2017,we conducted a survey among 1 014 nursing students in Medical School of Yangzhou Polytechnic College;then 488 students with left-behind experience were selected and assigned randomly into an intervention group receiving a 5-week mindfulness training and a control group without intervention at all.Five Facet Mindfulness Questionnaire (FFMQ),Symptom Checklist 90 (SCL-90),and Acceptance of Others (AOO) were used to evaluate psychological status,the mindfulness level,and interpersonal acceptance ability of the participants of the two groups.Results Before the intervention,there were no significant differences in overall and dimensional scores of FFMQ,SCL-90 and AOO scales between the participants of intervention group and control group (P>0.05 for all).Compared to those in the participants of control group,significantly lower SCL-90 dimensional scores for obsessive symptoms (1.13±0.74 vs.1.50±0.75),interpersonal sensitivity (1.33±0.74 vs.1.52±0.78),depression (1.15±0.57 vs.1.41±0.70),anxiety (1.06±0.60 vs.1.25±0.78),hostility (0.49±0.56 vs.0.79±0.78),phobic anxiety (0.56±0.43 vs.0.83±0.71) and paranoid ideation (0.61±0.54 vs.1.08±0.59) were observed in the participants of mindfulness training group after the intervention (P<0.05 for all);the participants of intervention group also showed significantly higher overall FFMQ score (124.13±7.79 vs.113.94±11.09) and FFMQ dimensional scores of act-aware (29.97±3.92 vs.26.46±5.23) and non-reacting (21.20±3.71 vs.18.71±3.26),and total score of AOO (63.04±6.17 vs.57.48±9.87 ) than those of control group (all P<0.05).Conclusion Mindfulness intervention can effectively improve negative emotion and interpersonal acceptance ability of nursing trainees with left-behind experience and the intervention could promote the establishment of good nurse-patient relationship.