Abstract: Objective To analyze influence factors of hand,foot and mouth disease (HFMD) among kindergarten children and to provide references for HFMD control and prevention.Methods A hand hygiene intervention was conducted in 18 kindergartens selected with stratefied random cluster sampling in Shenzhen city in 2015.The basic information of all the 8 275 children in the kindergartens were collected with a questionnaire survey and the HFMD incidents in the children were monitored from April to October in 2015.Results Among the kindergarten children,totally 122 HFMD cases were diagnosed,of which 9 children were recurrent cases,during the monitoring period.Single factor analysis showed that the HFMD incidence in the kindergarten children of intervention group was significantly lower than that in those of the control group (0.9% vs.2.5%,χ²=33.154; P<0.001); the incidence of HFMD showed a reverse correlation with the age among the children; and the children living in residential communities had a higher HFMD incidence than those in urban villages (1.7% vs.0.5%,χ²=14.019; P<0.001).Multivariate analysis showed that hand hygiene intervention could significantly reduce the HFMD incidence in the kindergarten children (odds ratioOR=0.404,95% confidence interval95% CI:0.272-0.601); the children at younger ages,with lower familial income,living in residential communities,and with guardians received higher education were more likely to have HFMD incidence.Conclusion The hand hygiene intervention could reduce HFMD risk in kindergarten children and HFMD incidence is manily influenced by age,family income,living area and guardian's education background in the children.