Abstract: Non targeted mutagenesis refers to the mutagenesis that happens at undan aged base sites of DNA so as to differ fmm targeted mutagenesis and its mechanisns in manmalian cell is tanaint obeveri fied Inthisresear thedy name charachteristic;of nontargeted mutagenesis induced with chanicalm utagen MNNG in A frica green monkey kidney veroellsw as illustrated fiistfolb weel by blocking gene transor tpnand transhtion with RNA and protein synthetic;inhibitor respective at the tinapoint generated the highest level of non targeted mutation.