Effect of curcumin on apoptosis and its mechanisms in SW872 adipocytes

Objective To explore the effect of curcumin on the apoptosis of SW872 adipocytes and the molecular mechanisms of the apoptosis. Methods The proliferation potential of SW872 preadipocytes and mature adipocytes treated with different concentrations of curcumin were evaluated by methyl thiazol tetrazolium(MTT)methods.The apoptosis of SW872 mature adipocytes were determined with 4',6-diamidino-2-phenylindole(DAPI)staining.Western blot was used to detect the expression of poly(ADP-ribolse)polymerase(PARP)cleavage and Bax. Results Curcumin could significantly inhibit the replication of SW872 preadipocytes in a dose-and time-dependent manner.Curcumin could inhibit the viability of SW872 mature adipocytes in a dose-and time-dependent manner.We observed nuclear shrinkage and deformation in SW872 mature adipocytes by DAPI staining.Curcumin could cause the increases in PARP cleavage and Bax expression in a dose-dependent manner. Conclusion Curcumin mediates adipocyte apoptosis via a mitochondrial pathw ay in SW872 adipocytes.