Effect of phenylmethyl sulfonylfluoride(PMSF) on total differential expression proteins in the spinal cord tissue of TOCP-treated hens

Objective To screen differential expression proteins in the lumbar spinal cord tissue of triorthocresyl phosphate(TOCP)-treated hens and to provide evidence for mass spectroscopy.Methods Twenty-four adult Roman hens were randomly divided into three groups and 8 hens in each group.The hens in TOCP group were treated with a single oral dosage of 1 000 mg/kg TOCP.The hens in the PMSF intervention group first were given 40 mg/kg PMSF and then treated with 1 000 mg/kg TOCP 24 hours later.The control group received the same volume of sodium chloride.On the 5th day after the exposure,the hens were sacrificed and their lumbar spinal cord tissue were dissected and homogenized in ice bath.Total proteins of the lumbar spinal cord tissue were separated by isoelectric focusing as the first dimension and sodium dodecyl sulfate-ployacrylanide gel electrophoresis(SDS-PAGE)as the second dimension.The two-dimensional electrophoresis maps