protective effect of aduola fuzhenglin on immune injury induced by microwave exposure in rats

Objective To investigate the protective effect of aduola fuzhenglin(ADL)on immune injury induced by microw ave exposure in rats.Methods Totally 140 male Wistar rats were randomized into control,radiation,and ADL group and treated with 0.75,1.5,and 3g/kg ADL,respectively,for 14 days before they were exposed to 30mW/cm² microw ave radiation for 15 minutes.The counts of leucocytes and lymphocytes,ratio of CD4⁺/CD8⁺,levels of interleukin-2(IL-2)and interleulkin-4(IL-4)in peripheral blood were detected with cell count,flow cytometry,and radioimmunoassay at 7th day and 14th day after the microw ave exposure.Results Seven days after the exposure,the ratio of CD4⁺/+CD8⁺decreased from 2.13±0.10 in control group to 1.74±0.17 in radiation group(P<0.01)and was significantly lower than that of in 0.75 g/kg group(2.15±0.16)and that of in 3g/kg group(2.09±0.31)(P<0.05).Fourteen days after the exposure,the counts of leucocytes in radiation group decreased from 7.17±1.09×10⁹/L in control group to 5.82±0.72×10⁹/L in radiation group(P<0.05)andwere lower than that of in 1.5g/kg group(9.09±1.84×10⁹/L)and that of in 3 g/kg group (8.61±1.86×10⁹/L)(P<0.05).The ratio of CD4⁺/CD8⁺in radiation group was 1.70±0.15 and was lower than that of in control group(2.06±0.31,P<0.05)and that of in 1.5g/kg group(2.05±0.34)in 3g/kg ADL group(2.36±0.21; P<0.05).The levels of IL-2 and IL-4 were not significantly different among the 5 groups after the exposure.Conclusion Treatment with 3g/kg ADL for 14 days is approved to protect against immune injury caused by microw ave exposure in rats.