Effects of genistein on cell cycle of human glioma cell line U251MG

Objective To investigate the effects of genistein on the growth and cell cycle of human glioma cell line U251MG.Methods The growth inhibition of U251MG cells was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide(MTT)assay.Phase distribution of cell cycle was analyzed with flow cytometry.The expression of cyclin B1 and cyclin-dependent protein kinase 1(CDK1)were determined with western blotting.Results Genistein inhibited the grow th of U251MG cells significantly in a dose-and time-dependent manner.When U251MG cells were treated with genistein for 48 hours,the cell cycle was arrested in the G2₂/M phase and cyclin B1,CDK1 protein expressions were significantly decreased in a dose-dependent manner(P<0.01,P<0.01).Conclusion Genistein can inhibit the grow th of U251MG cells significantly.G2₂/M arrest of U251MG cells after treated with genistein may be related to the decreased protein expression of cyclin B1 and CDK1.