Screen and identification of oil-degrading bacteria from oil polluted soil in Xingjiang

Objective To isolate and identify petroleum-degrading bacteria from oil-polluted soil in Karamay of Xinjiang Uyghur Autonomous Region.Methods By enriching the polluted soil with petroleum hydrocarbons as the only carbon source,the oil-degradation bacterium from oil-contaminated soil was screened and identified by its 16S rDNA sequencing. Then basic local alignment search tool(BIAST)was used to indicate the sequence of the isolates from the GenBank.Results Totally 18 bacteria strains were isolated and identified from oil-polluted soil.The sequence of its 16S rDNA indicated that there were 98% of homology to the representative strains.The main strain were Pseudomonas sp.,Planococcus sp., Arthrobacter sp.,Psychrobacter sp.,Brevibacillus agri sp.,and Brevundimonas sp..The bacteria strains from different oilpolluted soil were different and there were more bacteria strains in heavy oil-polluted soil.Conclusion The main strain was Pseudomonas sp.in oil-polluted soid and the bacteria strains were different in different oil-polluted soil.