Effects of bisphenol A on calcium channel currents in dorsal root ganglion neurons of rats

Objective To explore the effects of bisphenol A(BPA)on calcium currents in dorsal root ganglion(DRG) neuron cells of rats. Methods Ca²⁺ currents were obtained in DRG neurons by whole-cell patch clamp technique;calcium transients induced by 50 mmol/L KCl were also observed in the neurons with laser scanning confocal microscopy assay,and the effects of bisphenol A on calcium channels were observed. Results BPA at the concentrations of 0.1,1,10,and 100 μmol/L significantly inhibited calcium current in DRG neurons cells,with an IC 50 of 11.41μmol/L.BPA shifted the activation curve of calcium currents toward a depolarizing direction and increased the slope factor of the curve.Additionally, 10μmol/L BPA inhibited the increment of intracellular Ca²⁺ concentration(Ca²⁺i)induced by 50 mmol/L KCl. Conclusion BPA has significant inhibitory effects on calcium current and calcium transient invoked by KCl in dorsal root ganglion neurons.