Cloning expression,purification and characterization of cashew nut allergen Anao2

Objective To clone and express the gene of the major allergen Anao2 from cashewnut.Methods The open reading frame(ORF)of Anao2 was cloned and inserted into the expression vector pMAL-c.The vector was transformed into Escherichia coli BL21(DE3)and the protein expression was induced by isopro-pyl-,-D-thiogalcatopyranoside(IPTG).Results The cloned ORF containing 1 332 bp and encoding 443 amino acids was authenticated to be Anao2.The recombinant Anao2 protein induced by IPTG was consistent with the actual value.Conclusion Cashew recombinant Anao2 protein is obtained.