Potective effect of geranylgeranylacetone on multiple organ injury in heatstroke rats

Objective To examine whether geranylgeranylacetone(GGA)could attenuate heat-induced multiple organ dysfunction in rats.Methods Fifty female anesthetized Wistar rats of clean degree were randomly divided into normothermic control group(NT,n=10),heatstroke model group(HS,n=10),lowdose GGA group(GGA1,n=10), moderate dose GGA group(GGA2,n=10),and high dose GGA group(GGA3,n=10).The model and GGA groups were orally administered vehicle(arabic gum)or GGA of 50 mg/kg,100 mg/kg,and 200 mg/kg,respectively,followed by the exposure to heat(43℃ for 70 min)before recovery at room temperature(RT,24℃).The normal control group rats were treated with vehicle and were kept at RT.A DKB-501S high precision water bath and circulation chamber of water were used to establish heatstrake model.The environment temperature was kept at 22-24℃ and the water temperature was kept at 45.0℃ for more than one hour,and then the temperature in the chamber was kept at 43.0℃.Results Compared with normothermic controls,all vehicle-treated heatstroke rats displayed hepatic and renal dysfunction,including increased levels of serum urea nitrogen,creatinine,aspartate aminotransferase,and alanine aminotransferase.The heat-stress response indicators were all significantly suppressed by GGA pretreatment.The pathological changes of lungs in heatstroke rats were alos attenuated by GGA pretreatment.Conclusion GGA preconditioning attenuates heat-induced multiple organ injury in rats.