Establishment of a loop mediated isothermal amplification assay for detection of Hantaan virus

  Objective  To establish a new method for rapid and efficient detection of Hantaan virus with loop mediated isothermal amplification (LAMP) technique.

  Methods  Four specific primers were designed and synthesized according to conserved region of Hantaan virus S fragment and a LAMP detection method was established.

  Results  The established method can effectively detect Hantaan virus standard plasmid and the primers have good specificity for the detection of Hantaan virus. The detection sensitivity of LAMP is 10 copies per reaction. And the established LAMP method can detect Hantaan virus in blood samples effectively.

  Conclusion  The established LAMP method is highly sensitive and specific, and it is an efficient and rapid method for the detection of Hantaan virus.