Objective To explore the relationship between tumor necrosis factor receptor superfamily 4 (TNFRSF4) rs17568 and tumor necrosis factor superfamily 4 (TNFSF4) rs3850641 gene polymorphism and hepatitis C virus (HCV) infection and to provide clinical evidences for screening, diagnosis and prevention of HCV infection.
Methods From October 2011 to May 2015, we conducted a study in Jiangsu province among 1 269 HCV antibody-positive cases and 1 691 HCV antibody-negative controls; the recruited participants included 749 renal dialysis patients (168 cases and 581 controls) in 9 hospitals, 1 741 previously paid blood donors (810 cases and 931 controls) from 20 natural villages and 470 intravenous drug users (291 cases and 179 controls) in a mandatory detoxification center sponsored by Nanjing Municipal Public Security Bureau. Fasting venous blood samples (5 mL for each) were collected from all the participants for the genotyping of TNFRSF4 rs17568 and TNFSF4 rs3850641 with TaqMan-MGB probe real-time fluorescent quantitative PCR method. Univariate and multivariate logistic regression models were used to analyze the relationship between TNFRSF4 rs17568 and TNFSF4 rs3850641 gene polymorphisms and HCV infection.
Results In the cases, TNFRSF4 rs17568 AA, AG and GG genotype carriers accounted for 46.69%, 43.05% and 10.26%, while in the controls those genotype carriers accounted for 44.87%, 44.44% and 10.68%; the TNFSF4 rs3850641 AA, AG and GG genotype carriers accounted for 66.09%, 30.53% and 3.37% in the cases but accounted for 71.89%, 26.01% and 2.09% in controls, respectively. After adjusting for gender, age, alanine aminotransferase (ALT) abnormality, aspartate aminotransferase (AST) abnormality and route of HCV infection, unconditional multivariate logistic regression analysis demonstrated that the individuals carrying TNFSF4 rs3850641 AG/GG genotype were more susceptible to HCV compared with those carrying TNFSF4 rs3850641 AA genotype (codominant model: odds ratio OR = 1.251, 95% confidence interval 95% CI: 1.052 – 1.488, P = 0.011; dominant model: OR = 1.275, 95% CI: 1.077 – 1.509, P = 0.005); the individuals with rs3850641 G allele had an increased risk of HCV infection (additive model: OR = 1.251, 95% CI: 1.078 – 1.450, P = 0.003). No correlation was observed between TNFRSF4 rs17568 gene polymorphism and HCV infection (all P > 0.05).
Conclusion TNFSF4 rs3850641 gene polymorphism is associated with HCV infection.
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