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Volume 38 Issue 6
Jun.  2022
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ZHANG Xiao-hong, HUANG Zhi-miao, ZHU Ying, . Effect and mechanism of miR-135b on apoptosis of HCT116 colon cancer cells[J]. Chinese Journal of Public Health, 2022, 38(6): 783-786. doi: 10.11847/zgggws1134110
Citation: ZHANG Xiao-hong, HUANG Zhi-miao, ZHU Ying, . Effect and mechanism of miR-135b on apoptosis of HCT116 colon cancer cells[J]. Chinese Journal of Public Health, 2022, 38(6): 783-786. doi: 10.11847/zgggws1134110

Effect and mechanism of miR-135b on apoptosis of HCT116 colon cancer cells

doi: 10.11847/zgggws1134110
  • Received Date: 2021-01-26
    Available Online: 2021-12-30
  • Publish Date: 2022-06-01
  •   Objective  To explore the effect and mechanism of microRNA-135b (miR-135b) on apoptosis of HCT116 colon cancer cells.   Methods   HCT116 cells were divided into control group, miR-135b NC group and miR-135b inhibitor group. Cell viability was detected with 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Cell apoptosis was detected with Hoechst staining. The expression of B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X (Bax), poly ADP-ribose polymerase (cleaved-PARP), cysteinyl aspartate specific proteinase (cleaved-caspase 3), phosphatase and tensin homolog deleted on chromosome ten (PTEN), phosphatidylinositol 3-kinase (PI3K), and protein kinase A (AKT) were detected with Western blot.   Results  The expression of miR-135b was down-regulated in miR-135b inhibitor group compared with that in control group and miR-135b NC group (0.53 ± 0.05 vs. 1.00 ± 0.03 and 1.02 ± 0.04) (F = 259.19, P < 0.05). The cell viability and apoptotic rate of HCT116 cells were 0.58 ± 0.05 and 3.24 ± 0.13% for control group, 0.59 ± 0.06 and 3.27 ± 0.08% for miR-135b NC group, and 0.39 ± 0.04 and 36.48 ± 0.52% for miR-135b inhibitor group, respectively, with significantly decreased viability but increased apoptotic rate of HCT116 cells for miR-135b inhibitor group compared with that for control group and miR-135b NC group (both P < 0.05). Significantly down-regulated expressions of Bcl-2, cleaved-caspase 3, PI3K, and p-Akt and up-regulated expressions of Bax, cleaved-PARP, and PTEN were detected in miR-135b inhibitor group in comparison with those in control group and miR-135b NC group was (P < 0.05 for all).   Conclusion   Dow-regulation of miR-135b could induce apoptosis of HCT116 cells, which might be related to the blocking of PTEN/PI3K/Akt signaling pathway.
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  • [1]
    Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA: A Cancer Journal for Clinicians, 2018, 68(6): 394 – 424. doi: 10.3322/caac.21492
    [2]
    Thanikachalam K, Khan G. Colorectal cancer and nutrition[J]. Nutrients, 2019, 11(1): 164. doi: 10.3390/nu11010164
    [3]
    Ng C, Li HJ, Wu WKK, et al. Genomics and metagenomics of colorectal cancer[J]. Journal of Gastrointestinal Oncology, 2019, 10(6): 1164 – 1170. doi: 10.21037/jgo.2019.06.04
    [4]
    Dawson H, Kirsch R, Messenger D, et al. A review of current challenges in colorectal cancer reporting[J]. Archives of Pathology and Laboratory Medicine, 2019, 143(7): 869 – 882. doi: 10.5858/arpa.2017-0475-RA
    [5]
    Saberinia A, Alinezhad A, Jafari F, et al. Oncogenic miRNAs and target therapies in colorectal cancer[J]. Clinica Chimica Acta, 2020, 508: 77 – 91. doi: 10.1016/j.cca.2020.05.012
    [6]
    Liu GK, Li BQ. Role of miRNA in transformation from normal tissue to colorectal adenoma and cancer[J]. Journal of Cancer Research and Therapeutics, 2019, 15(2): 278 – 285.
    [7]
    Li L, Wang AL, Cai M, et al. Identification of stool miR-135b-5p as a non-invasive diaognostic biomarker in later tumor stage of colorectal cancer[J]. Life Sciences, 2020, 260: 118417. doi: 10.1016/j.lfs.2020.118417
    [8]
    Wu CW, Ng SC, Dong YJ, et al. Identification of microRNA-135b in stool as a potential noninvasive biomarker for colorectal cancer and adenoma[J]. Clinical Cancer Research, 2014, 20(11): 2994 – 3002. doi: 10.1158/1078-0432.CCR-13-1750
    [9]
    袁平, 张小鸿, 王家兴, 等. 血清miR-135b在结直肠癌中的表达及临床意义[J]. 慢性病学杂志, 2019, 20(8): 1121 – 1124.
    [10]
    陈明倩, 马莉, 滕银成. miR-135b在肿瘤发生发展中的研究进展[J]. 医学综述, 2018, 24(24): 4836 – 4841. doi: 10.3969/j.issn.1006-2084.2018.24.010
    [11]
    Chen Z, Gao YJ, Gao SH, et al. MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Kruppel-like factor 4 (KLF4)[J]. Archives of Medical Science, 2020, 16(1): 167 – 176. doi: 10.5114/aoms.2019.87761
    [12]
    Gao JS, Zhang L, Liu ZA, et al. Effect of miR-135b inhibitor on biological characteristics of osteosarcoma cells through up-regulating PPM1A[J]. International Journal of Clinical and Experimental Pathology, 2019, 12(3): 689 – 699.
    [13]
    Hu ML, Zhu SX, Xiong SW, et al. MicroRNAs and the PTEN/PI3K/Akt pathway in gastric cancer (Review)[J]. Oncology Reports, 2019, 41(3): 1439 – 1454.
    [14]
    Frankson R, Yu ZH, Bai YP, et al. Therapeutic targeting of oncogenic tyrosine phosphatases[J]. Cancer Research, 2017, 77(21): 5701 – 5705. doi: 10.1158/0008-5472.CAN-17-1510
    [15]
    Chen CY, Chen JY, He LN, et al. PTEN: Tumor suppressor and metabolic regulator[J]. Frontiers in Endocrinology (Lausanne), 2018, 9: 338. doi: 10.3389/fendo.2018.00338
    [16]
    Álvarez-Garcia V, Tawil Y, Wise HM, et al. Mechanisms of PTEN loss in cancer: It's all about diversity[J]. Seminars in Cancer Biology, 2019, 59: 66 – 79. doi: 10.1016/j.semcancer.2019.02.001
    [17]
    Costa RLB, Han HS, Gradishar WJ. Targeting the PI3K/AKT/mTOR pathway in triple-negative breast cancer: a review[J]. Breast Cancer Research and Treatment, 2018, 169(3): 397 – 406. doi: 10.1007/s10549-018-4697-y
    [18]
    Marquard FE, Jucker M. PI3K/AKT/mTOR signaling as a molecular target in head and neck cancer[J]. Biochemical Pharmacology, 2020, 172: 113729. doi: 10.1016/j.bcp.2019.113729
    [19]
    Wu YY, Hu G, Wu RL, et al. High expression of miR-135b predicts malignant transformation and poor prognosis of gastric cancer[J]. Life Sciences, 2020, 257: 118133. doi: 10.1016/j.lfs.2020.118133
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    • Receive:  2021-01-26
    • Online:  2021-12-30
    • Published:  2022-06-01

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