Studies the Changs of Leptin and Hormones During Induce the Obesity of Rats

To study the change of leptin with westhern blot and detect the insulin(INS)and growth hormone(GH)in serum using radioimmunoassay during high-calorie diet induce rats obesity.The results show leptin and INS were rised with increased bodyweigh (BW)of rats,but the GH was declined with the increased BW.It suggest that there are relations between obesity and leptin and exist mutual action mechanism among leptin and insulin and GH during inducing rats obesity.