Inhibition Roles of Selenium on Extracellular Matrix of Hepatocytes and Ito Cells Injured by Carbon Tetrachloride

To study the inhibitory effects of Selenium(Se)on extracellular matrix(ECM)production of hepatocytes and ito cells in vitro,rat hepatocytes and ito cells were isolated and cultured.The results showed that addition of carbon tetrachloride to hepatocytes and ito cells pretreated with 0.36 nmol/L Se 4 hours before,the incorporation of ³H-proline and the secretion of precollagen type Ⅲ and the production of malondialdehyde were markedly inhibited and the activity of glutathione peroxidase(GPx)obviously increased.Se also inhibited the excretion of hyaluronic acid、laminin and fibronectin in cultured ito cells.The analysis of correlation indicated that the effects of Se on antioxidative injury and ECMs production depended mainly on increasing the activity of GPx.