Experimental Study on Apoptosis of Lung Tissues in Rats Exposed to COF
-
Abstract
SD rats were exposed to subacute dose cooking oil fumes (COF) in order to observe p53 bcl-2, bax protein level of Lung tissues. Rats of test group and negative group inhaled COF(43±4mg/m3×30min/d×20, 40, 60d) and hot air (30min/d×20,40, 80d) respectively,while positive control group was droped B (a) P from trachea(12mg/kg/5d×20,40,60d). An immunohistochemical study was made to investigate the expression level of P53 gene. In the study,the level P53 of COF and B(a)P group are higher than control group. A close timeeffect relationship was existed in COF group bcl-2 protein level was as like as p53 protein. The level of p53、bcl-2 protein of adenic metaphasin arcad precancerous lesion tissues were higher than other lung tissues,wheras the level of bax protein was lower than control group. This suggested, the expression of apoptosis related to genes may play role in the pathological changes exposed to COF.
-
-