Establishment and Application of Immuno-PCR for the Detection of HBsAg

ObjectiveImmuno-PCR was established.Sensitivity、specifity and repetition were detected in standard of HBV, comparing with ELISA.ResultsThis assay offer a senitivity of 1pg/ml,800 fold more sensitive than conventional ELISA,the positive percentage of ser um HBsAg in 51 cases of HB patients was 17.6% (9/51).ConclusionImmuno-PCR for serum HBsAg is a high specificty、sensitivity and repetition.It is significant in clinical diagnosis treatment and prognosis of HB.