Effect of Cholesterol Defeciency on the Activity of Protein Tyrosine Kinases in Jurkat Cells

Objective and Methods In this report,the molecular mechanisms of cholesterol deficiency on the proliferation of T lymphocytes was investigated from the aspect of protein tyrosine kinases(PTKs)signal transductions by using of the methods of ³HTdR incor porationtest,indirect immunofluorescent stain,FCM and ICC stain in Jurkat cells.Results The results showed that cells proliferation were inhibited and blocked on G₀/G₁ phase and the activity of PTKs were decreased in Jurkat cells after treated with lovastatin for 3 days.When treated with LDL,they could partly increased.Conclusions These results suggest that the decrease of the activity of PTKs may be related to the effects of cholesterol deficiency on proliferation of Jur kat cells.