Study On Aetiology of Rickettsia Heilongianggii Tick Borne Spotted Fever

Objective To study the aetiology and epidemiology of tick borne spotted fever in northeastern area.Method Monoclonal antibodies,immunohlot-ting,endonuclease restiction analysis of DNA,PCR/RFLP.PCR/SSCP and DNA sequencing were used.Results (1)The results demonstrated that R.heilongjiangii was different from other international reference strains of SFGR and was proposed to be a new species of SFGR.(2)R.heilongjiangii and H-5 strain from a Patient's blood specimen were same species,which has ability to cause disease in human.Conclusion The investigation with epidemiology demonstrated that Dongning and Luobei of Heilongjiang province and Hunchun area of Jilin province are the nature foci of R1heilongjiangii tick borne spotted fever.