Study on Several Toxigenes Detection of Vibrio Cholerae

Objective To realize the positive rates of several main toxigenes in the strains of vibrio cholerae isolated in Zhejiang Province.Methods Labeling toxigene probes with Digox igenin,91 strain of vibrio cholerae isolated from Zhejiang Province for toxigene were detected.Results To xigenes such as ctxA B,Zot Ace generally existed in epidemic strains of EVC and VCO₁₃₉,positive rate of these three genes were separately 100%,98.86% and 96.59%;these three genes did not ex ist in non-epidemic strains of EVC.Conclusion It was showed that more attention should be paid to surveillance and study of epidemic EVC and VCO₁₃₉.Restriction fragment length polymor phism hybridization analysis of ctxA B gene indicated that VCO₁₃₉ was similar to epidemic EVC in hereditary characteristics,there were two copies in both of them.