Expression of Human Papillomavirus Type 16 Capsid Proteins in Baculovirus-insect Cell System

Objective To construct the recombinant baculoviruses containing L1 or L1 Plus L2 genes and express high-efficiency capsid proteins in insect cells.Methods By co-transfection of linearized baculovirus DNA and recombinant baculovirus transfer plasmids containing L1 or L1+L2 respectively,two recombinant baculovir us strains were gotten.Results The L1 and L2 genes of capsid protein were amplified by PCR in recombinant baculovirus;The 57KD L1 protein and the 95 KD,110KD L2 proteins were detected by Coomassie-blue staining and Western-blot;ME studies showed that there were the HPV16 virion in sf9 cells.Conclusion These results would be the basis to develop the HPV 16 prophylactic vaccines and diagnosis kit.