Study on Influence Factors of Formaldehyde Fumigation in Destroying HBsAg According to Orthogonal Design

Objective The for maldehyde fumigation experiment was conducted to evaluate the influence of fumigation hours,fumigation dose(g/m³),temperature(℃)and relative humidity(%)in destroying HBsA g according to or thogonal design of L₉(3⁴.Methods(1)Purification HBsAg was used as evaluation index.(2)Round stainless steel,emulsion plates with diameter of 1.0cm,and square cloth plates with 1.5cm 1.5cm were prepared as carriers.(3)For maldehyde gas was produced from parafor maldehy deoxidized by potassium permanganate.Results The fumigation hour was mostimpor tant influence factor in destroying HBsAg,next were paraformaldehyde dose(20g/m³),temperature(40)and relative humidity(55%)(F=493.93,124.43,46.00,120.43,P<0.01).