Aetiological Analysis and Study on E.coli O157：H7 in Epidemic Area

Objective To analyze E.coli O157:H7 of epidemic area.Methods To compare the virulence gene spectrum of E.coli O157:H7 strains by PCR and analyze the homology of E.coli O157:H7 strains by PFGE and RAPD.Results In all E.coli O157:H7 strains isolated from epidemic area,100% possess Hly and eaeA gene,95.35% possess SLT2 gene,11.63% possess SLT₁ gene.The PFGE spectrum shows that the strains isolated from epidemic area are distinctively different from the strains isolated from Japan,and they are similar to but not same with the standard strain 882364.The PFGE spectrum of strains isolated from epidemic area patients are same with that of strains isolated from excrement of prutries and domestic animals and inscet intestine.Conclusion Poultries and domestic animals which carry E.coli O157:H7 could be source of infection.PFGE could be used to analyze E.coli O157:H7 and is important in epidemicology study,but is not fit to be used in grass roots unit.Analyzing E.coli O157:H7 by RAPD is convenient and time saving.