Study on Circulating Immune Complexes of IgG Specific Activing Complement in Patients with Graves’ Disease

ObjectiveTo explore existing state and its pathology meanings of the two component circulating immune complexes of IgG specific activing complement(IgG/C₃-TCIC)in patients with Graves'disease(GD).MethodsBy a capture EL ISA,used coat IgG anti-human C 3 for coated antibody,HRP-coat anti-human IgGfor second antibody,taking 219 cases of GD as a test group and 105 healthy individuals as a control one,IgG/C₃-TCIC were characterized in this paper to compare its positive rate between the normal and abnormal levels of serum Ig,C₃,T₃,T₄,FT₃,FT₄,TG,TM and different age, sex,and to analyse the correlation between IgG/C₃-TCIC and serum total,free,composed Ig and C₃.ResultsBoth the levels and positive rates of IgG/C₃-TCIC in GD patients were remarkably higher than those in the controls(P<0.01,0.001), its positive rates were no relations with age,sex and the levels of serum thyroxine,only notablely higher in the levels of serum IgA than those of the normal groups(P<0.05).IgG/C 3-TCIC in GD patients only correlated with the complexed IgM(r=0.173 2,P<0.05).In contrast,IgG/C 3-TCIC correlated with total,free IgM and complexed C₃(r=0.496 8,0.473 1, 0.211 3,P<0.001,0.001,0.05).ConclusionThere were specific immune disorder in patients with Graves'disease.Ig switch had an important effect on regulating the formation of IgG/C₃-TCIC.It cut a new approach to further illustrate the mechanism of the relational autoimmune diseases.