Analysis on Single-Strand-Conformation Polymorphism of 16S rRNA Gene of Five Species Food-borne Pathogenic Bacteria

Objective To detect five species food-borne pathogenic bacteria Salmonellaty phimurium,Staphylococcus aureus,Escherichia coli,Shigellosis Flex neri Bacillus cereus to analyse single-strand-conformation polymorphism(SSCP)of the 16Sr RNA gene,and investigate the possible role of this technology at rapid indentification of bacteria.Methods After the number of five bacter ia strains of food poisoning was increaed,the bacteria DNA was extracted.A pair of special primers for amplification located in 16SrRNA sequence flanking a variable region were designed,and analysis of the amplified product was based on the principle of SSCP.Results All the strains separ ated two or more special bands,and the distances and relative position of band pattern were different from each other but with good repetition.The sensitivity of this technique obtained for Salmonella typhimurium was 10-15 CFU.Conclusion The 16S rRNA gene of five pathogenic bacteria strains by SSCP assay were species-specific patterns,this technique was helpful to rapid,accurate,molecular identification of pathogenic bacteria of food poisoning.