Study on Coexpression of Env/IFNα-2b Fusion Gene in Vaccinia Virus

Objective pJ16 was used expression vector,Env gene encoding type Ⅰ envelope protein of AIDS virus(HIV) and encoding interferon α-2b gene of human were inserted on the downstream of the hybid promotor,where HA gene of the vaccinia virus unnecessary areas was the flank and there were 16 p7.5 promtors of mutant-type vaccinia virus.Methods A-type inclusion later promoter of vaccinia virus and vJ-env-IFNα-2b was acquired by homologous recombination with the natural vaccinia virus an selection of the HA-plaque as substrate.Results The analysis of IFA.dot blot,SDS-PAGE,Western blot and Dot-ELISA.That the recombinant viruses could express Env/IFNα-2b,whose molecular weight was 100kDa.In the mice immunization expreiment,the OD value of the serum antibodies to HIV-1(P<0.001) in groups.There was difference between contain IFN and vJ16env.Conclusion The expressed env/IFNα-2b proteins both had good reactinogenicity and immunogenicity.