Analysis on gene type of isolated strains of orientia tsutsugamushi from Heilongjiang area by way of PCR and RFLP

  Objective   To determined the gene type of isolated strains of orientia tsutsugamushi(Ot)in Heilongjiang area.

  Methods   PCR and RFLP were used to analyse their restriction maps of sta 56 gene of the isolated strains and made a comparison between the isolated stains of Ot and the inter national reference strains.

  Results   The distinctive PCR products of the 6 isolated strains from Heilongjiang areas through restriction with HinfⅠ, HhaⅠ, RsaⅠ appeared three different kinds of RFLP restriction maps.The strains MSAa9301, MSAa9303, MSAa9304, MSAp9305 had the similar restriction map with reference strain Gilliam, but they did nothave restriction spot of HinfⅠ, may be classified into the same type; strains MSAa9306 had the same restriction map with reference strain karp, may be classified into the same type; strains MSAP9302 had the same restriction map with reference strain kato, may be classified into the same type.

  Conclusion   At last three types: Gilliam, Karp and Kato were exsited in the separated specise six Ot of Heilongjiang area.