Toxicity effects of manganese on growth and cell cycle of SN4741 cells

  Objective   To study the effects of different doses manganese on neurocyte, SN4741.

  Methods   SN4741 was incubated in culture media with 200, 400, 600, 800μmol/L manganese(MnCl₂)for 2 days, 4 days and 6 days respectively.The cell growth livingness was examed by MTT and cell growth curve was made.The cell cycle was monitor ed by flow cytometry(FCM), at the same time, morphological changes was investigated by transmisssion electron microscope.

  Results   Manganese of different concentrations(MnCl₂ 200, 400, 600, 800 μmol/L)could suppress the proliferation of SN4741 cells in dose and time-dependent manner.FCM showed that the cell cycle of the SN4741 cells could be inhibited in Speriod at certain observed through flow cytometry(FCM)and transmission electron microscope.

  Conclusion   The apoptosis of SN4741 cell induced by mang anese(MnCl₂)may be its important mechanism on proliferation inhibition.