Interaction between mutation of endothelial nitric oxide synthase gene and alcohol drinking in essential hypertension

  Objective   To analy ze the inter action between G894T(Glu298Asp)mutation in the exon 7 of endothelial nitric oxide synthase gene and alcohol drinking in essential hyper tension.

  Methods   One hundred and six teen essential hypertensives, screened from a steel factory workers, not taking hypertensive medication, and one hundred and thirty six normotensives, screened from health workers in the same factory were selected as the subjects in this study.Polymerase chain reaction (PCR)and restriction fragment length polymorphism(RFLP)were performed to detect the G894T mutation; Additive model was used to analyze the interaction; Population attributable risk percent(PAR%)was applied to calculate the etiologic fraction.

  Results   There was a positive interaction between G894T mutation and alcohol drinking in essential hypertension; Attributable interaction was 3.69;Attributable interaction percent was 48.68%;Pure attributable interaction percent was 561.08%.After adjusting age, sex; smoking, body mass index by logistic regression, there was also positive interaction between G894T mutation and alcohol drinking in essential hypertension; Attributable interaction was 1.13;Attributable interaction percent was 19.09%;Pure attributable interaction percent was 22.97%.Under certain conditions, PAR% was about 10%-15%.

  Conclusion   s The inter action between G894T mutation in the exon 7 of endothelial nitric oxide synthase gene and alcohol drinking had a positive effect on the essential hypertension in this studied population.To control the alcohol drinking in the population who had G894T mutation and alcohol drinking simultaneously would produce markedly decrease of the essential hypertension in general population.