Study on using DASS-FAT to detect clonorchiasis sinensis

  Objective   To search the use value of DASS-FAT in diagnosis of infection with clonorchiasis sinensis.

  Methods   By the external culture of clonorchiasis sinensis to obtain the ES antigen and join it to Sepharous-4B, using FAT to detect sera antigen of the patients with clonorchiasis sinensis.

  Results   The special antibody positive rate was 100% in sera of the patients with clonorchiasis sinensis.In the helath people, the cerebral cysticercosis, and the infection with intestinal worm, the positive rate was 2.04%(28/1372), 2.27%(2/88), and1.80%(1/55).The cross-reactive was nonsignificant(χ²=0.021, P>0.1).The negative rate was 77.78%(28/36)of the antibody in sera with clonorchiasis sinensis after treatment (12 months).

  Conclusion   Using DASS-FAT to detect the special antibody in sera of the patients with clonorchiasis sinensis had high specificity and sensitivity, and the cross-reactive was nonsignificant in uninfected with clonorchiasis sinensis. The method was simple, rapid and the result of the test was stable.It can be used in the diagnosis, the examination of the effect of treatment, epidemiology and the basic study in clonorchiasis sinensis.