Rapid biolu minescent technique to detect bacteria in meat

  Objective   To establish a method to dete mine the bacteria pollution of meat rapidly.

  Methods   The microbial ATP and the somatic ATP are measured by using ATP-biolu minescence technique and removing somatic ATP technique.The microbial ATP content is directly proportional to bacteria count.

  Results   The somatic ATP of raw meat must be removed, so the measurement of the micobial ATP content of raw meat takes bout 15 min.The somatic ATP of the cooked meat is released and hydrolyzed, so the detecting time of microbial content of these cooked cured foods can be reduced to 4 min.In the procedure applied detection limit is about 10³ cfu/g.

  Conclusion   The bacteria polluting situation of meat can be deter mined rapidly and timely by using A TP-biolu minescence technique.The technique can be widely used to foods sanitation.