Objective To evaluate of Latex fixation tests for detection of methicillin resistant staphylococcus aureus and to detect of staphylococcus aureus enterotoxin.
Methods 130 staphylococcus aureus isolates extracted clinically were classified into methicllin resistant staphylococcus aureus (MRSA) and methicllin sensitive staphylococcus aureus (MSSA) based on the antibiotics sensitive test, A latex fixation tests to detect penicillin-bindingprotein 2a, was compared with the MecA PCR for the detection of methicllin resistance in staphylococcus aureus and the detection of staphylococcus aureus enterotoxin by Enzyme linked immunosorbent assay test.
Results Of the 65 MecA gene positive isolates, 62 were positive by the latex fixation, and all of the 63MecA gene negative strains were negative by latex fixation tests. MRSA with enterotoxin were 67 strains, by comparison, MSSA were 19 strains.
Conclusion Latex fixation test was simple, fast and accurate for detection of MRSA and great emphasis should be on the detection of staphylococcus aureus enterotoxin in clinical laboratory.
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