Effects of estradiol on EA As release by deltamethrin in glial cell

  Objective   Using rat primary glial cells as model in vitro to investigate the effects of estrogen on the release of EAAs by deltamethrin.

  Methods   Using HPLC to detect the EAAs release of different 17β-estradiol dose level (10^-5, 10^-8, 10^-11 mol/L) in the glial cells treated with deltamethrin. Meanwhile, the estrogen receptor(ER)antagonist tamoxifen was used to investigate the mechanism of estrogen.

  Results   The Glu release in the glial cells was increased significantly by 2×10^-5 mol/L deltamethrin, and 10^-8 estradiol can partly block this release. The effect of 10^-8mol/L estradiol can not be antagonisted by tamoxifen.

  Conclusion   Estradiol showed a neuroprotective effect on the release of Glu by deltamethrin in the glial cells.