Cell apoptosis induced by ceramide in human colon cancer cells

  Objective   The effect of cell apoptosis induced by ceramide was investigated in human conlon cells(HT29).

  Methods   The curve of cell growth(MTT test)was used. Cell apoptosis was detected under fluorescent and electronic microscope and expressed bcl-2, Fas, p53 protein.

  Results   Ceramide inhibited remarkably the cell growth and induced cell apoptosis. The expression of Fas protein in HT-29 cells increased as the dose increased and the expression of p53 protein decreased as the dose increased.

  Conclusion   Ceramide can significantly inhibit the growth of HT-29 cells and induce apoptosis.