Investigation on dengue virus from Aedes albopictus field in Guizhou

  Objective   To study molecular epidemiology of dengue virus from Aedes albopictus field in Guizhou province.

  Methods   18 different population of Aedes albopictus larvals were collected in Guizhou. The total RNA from mosquito were extracted. The viral NS1 gene fragment were amplified with reverse transcriptase polymerase chain reaction(RT-PCR). And the products were digested with Mav I for typing. Dengue virus in mosquito was isolated with C6/36 cells. Then the viral antigen was detected by indirect immunofluorescence assay(IFA).

  Results   One strain of dengue virus was isolated with C6/36 cells from field Aedes albopictus in Mawei town. It was identified as dengue virus type 2 by IFA with monoclonal antibody and RT-PCR. Dengue virus type 2 or 4 RNA were detected in A edes albopict us field collected from Kaili, Huangguoshu and Zhenyuan areas in Guizhou, respectively.

  Conclusion   These findings highlighted the existence of a silent epidemic in Guizhou.