Objective To develop a sensitive, specific and rapid enzyme-linked immunosorbent assay for detection of ochratoxin A(OA). To develop rapid detection kits that possessing patent of our country.
Methods To produced hybridoma cell lines excreting monoclonal antibodies against OA by using B cell hybridom technique. And to obtain the monclonal antibodies against OA.
Results The indirect competitive inhibition enzyme-linked immunosorbant assay(ELISA)was developed for detection OA in rice and wheat. The limit of detection concentration of OA was 0.5 ng/ml. The linear range of standard curve was between 2-500 ng/ml, the linear equation was Y=-0.272X+1.07(r=0.997 8). The recovery of OA was between 79.0%-119.7%. The OA contamination level of rice and wheat that were sold in Beijing was surveyed by using the method. Contamination rate of wheat was 60.71%, the maximum level was 8.26μg/kg; the contamination rate of rice as 17.86%, the maximum level was 3.44μg/kg.
Conclusion The detection method was simple, fast sensitive and can meet the demand of practical work.
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