Combination toxicology of formaldehyde, trichloroethylene and alchlor with Chinese Hamster Lung Cells

  Objective   To study the combined action of formaldehyde, trichloroethylene and alchlor.

  Methods   50% inhibition concentration(IC₅₀)and 95% confidence limits of formaldehyde, trichloroethylene, alchlorand mixture(formaldehyde, trichloriethylene and alchlor)were assessed using Methyl thiazolyl tetrazolium(MTT)with Chinese Hamster Lung Cells.Combined action of above mixture was evaluated accordingto Finney model and Log istic Regression Method.

  Results   IC₅₀ of formaldehyde, trichloroethylene, alchlorand the mixture were 0.0226 mg/ml, 0.368 mg/ml, 0.589 mg/ml and 0.124 mg/ml respectively.The Qvalue was 11.08 and Parameter B4 from Logistic Regression Method showed no significant.

  Conclusion   Combination tox icolog y of above mixture(formaldehyde: trichloroethylene: alchlor=6:11:33)belonged to additive effect.