Study on SOD gene expression and SOD activity in aging model mice induced by D-galactose

  Objective   To investigate the changes of activity and expression of superoxide dismutase(SOD)in aging model mice induced by D-galactose.

  Methods   Kunming mice were randomly divided into three groups including aging model mice induced by D-galactose group, control group and three level of panax(low, middle and high)groups.Biochemical assay and reverse transcription polymerase chain reaction(RT-PCR)were adopted tomeasure changes of SOD activity and gene expression in different groups.

  Results   Activity and gene expression of SOD decreased significantly in aging model mice induced by Dgalactose than those of control mice(P < 0.01).Compared with aging model mice group, activity and gene expression of SOD increased in all groups of panax.And the increase was significant in middle level group(P < 0.01).

  Conclusion   The activity and gene expression of SOD decreased in aging model mice induced by D-galactose.Oral-intake liquid of ginsengroyal jully could lower the decreased of SOD's activity and gene expression.