Effects of EGF and genistein on proliferation of human breast cancer cell

  Objective   To observe the effect of genistein and epidermal growth factor(EGF)on the proliferation of human breast cancer cell MCF-7 in vitro.

  Methods   Cell growth status was observed with methyl thiazolyl tetrazolium (MTT)when it was cultured with different doses of genistein or EGF for different time respectively, or with definite dose of genistein and EGF.

  Results   The growth of MCF-7 was inhibited when it was exposed to genistein over 25 μmol/L seemed to be an optional dose.Cell growth was greatly promoted when it was exposed to EGF over 4.15 nmol/L, the more the dose and time became, the greater the promoting effect was, and 8.30 nmol/L seemed to be an optional dose.When MCF-7 was cultured with 50 μmol/L genistein and 8.30 nmol/L EGF simultaneously, an interaction effect was observed.The promoting effect of EGF was reversed by genistein.

  Conclusion   Genistein can inhibit the proliferation of human breast Cancer cell MCF-7 induced by EGF.