Objective To explore the effect of ant hocyanin on matrix metalloproteinases(MMP)expression and activity.
Methods Human umbilical vein endot helial cell were isolated and incubated with 5μg/mL rCD40L for 24 h.After that, the endot helial cell were incubated with 1μM, 10μM, 100μM C3G and P3G for another 24 h, respectively.
Methods of zymography and Western blotting were used to analyze MMP-1, MMP-2, MMP-9 activities and protein expression.
Results Ant hocyanin C3G and P3G inhibited MMP-1, MMP-2 and MMP-9 protein expression and their activities significantly.
Conclusion Ant hocyanin C3G and P3G inhibited MMP-1, MMP-2, MMP-9 protein expression and their activities.