Study on a high throughput method of screening adducted site of DNA adducts

  Objective   To develop a high throughput method of screening the binding site of DNA adducts.

  Methods   The human liver tumor HepG2 cells were first treated with lng/μl nitrogen mustard for 3h and total DNA was extracted, then serrial analysis of gene expression was used for reference to improve ligation mediated PCR to detect the adducted site by nitrogen mustard in the cell genome.

  Results   PCR product corresponded to 75 base pairs approximately.

  Conclusion   The design of the experiment is rational and feasible.