Cloning of ferritin H chain gene from human Jurkat T lymphocyte induced by arsenic trioxide

  Objective   To understand the differentially expressed genes and to explore the effects on mechanism of gene transcription in human T lymphocytes induced by arsenic trioxide.

  Methods   Human J urkat T cell line as cell model was treated by arsenic trioxide(5μmol/L, 24 h)in vitro.Then the differential expressed genes were cloned and the subtractive cDNA library from Jurkat T cell line was constructed by suppression subtractive hybridization.The PCR and sequencing techniques had been applied to testive clones.

  Results   The forward subtracted cDNA library from J urkat T cell line induced by arsenic trioxide was constructed.Sequencing analysis showed that there were two clones contained ferritin H chain in the forward subtractive cDNA library.

  Conclusion   Arsenic trioxide can induce the transcription of ferritin H chain in T lymphocytes, and the ferritin H chain may play certain role in the mechanism of anti-arsenical cytotoxicity.