Relationship between DDVP resistance of German cockroaches and related enzyme

  Objective   To study the relationship between the activities of acety choline esterase, 1-NA, glutathione Stransferase and DDVP resistance of German cockroaches(Blattella germanica).

  Methods   To measure the activities of en zymes above mentioned in different DDVP resistant stains of German cockroaches by using visible spectrophotometry and kinetic microplate methods, and to carry through the statistical analysis by SAS softw are.

  Results   With DDvp resistant levels increased in Germand cockroaches, AChEs inhibition ratio, 1-NA and glutathone Stransferase enhancd gradually.The distinction was remar kabkle on inhibition ratio of acety choline esterases activity and 1-NAs activity among resistant strains and sensitive strains when resistant coefficient equal or less than 2 8.Nevertheless, the distinction was not remarkable for the activity of glutathiones transferase.

  Conclusion   AChEs inhibition ratio and 1-NAs activity are proved to some values as biomarkers of German cockroaches DDVP resistance.